Here we have the result of a standard deviation projection across 160 lightfield images recorded from a larval zebrafish optic tectum injected with Oregon Green BAPTA-1 calcium sensitive dye. The fish was watching a small blue dot move left and right, which should cause a certain population of neural cells to fire, flooding these cells with calcium and thus increasing their fluorescence. The SD projection technique is intended to highlight those voxels which exhibit the most fluorescence change over time.
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