Novel Microarray Technologies Related on the Proteins and Nucleic Acids
Lu Zuhong, State Key Laboratory of Bioelectronics (Chien-Shiung Wu Laboratory)

As the project of sequencing the human genome has been completed, we have entered so-called post-genomic era. The major subjects in the post-genome era are the functional genomics, proteomics, presonal genetic diversity, and their applications. The integration and miniaturization of bioassay for the high-throughput and high sensitive detection on the levels of nuclear acids, proteins and the other small biomolecules are the tendency of the modern development in life science. There are also a great number of challenges and opportunities in biological researches and medical applications.

Microarray technology is revolutionizing biology and medicine due to its ability of high-throughput and rapid detection of a large amount of molecular information in biological system. The working principle of the microarray is based on the specific interactions between biomolecules. This presentation will introduce a method for fabricating unimolecular double-stranded DNA microarrays on solid surfaces, which were used to probe sequence-specific DNA/protein interactions. Here, several new methods are developed to manufacture the double-stranded DNA microarrays. The accessibility and specificity of the DNA-binding proteins binding to dsDNA microarrays were verified by binding fluourescence-labeled NF-κB. The dsDNA microarrays have great potential to provide a high-throughput platform for investigation of sequence-specific DNA/protein interaction, which provides a general technique for high-throughput DNA-protein or proteomic studies.

Furthermore, I will describe some other our microarray platforms based on DNA and proteins for the practical applications.

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