1. Movie S2 High Resolution

    00:18

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    Animation of the image acquisition process, showing a lattice light sheet (blue/green) intersecting a cell (gray) to produce fluorescence (orange). As the cell is swept through the light sheet, a…

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    • Movie S1 High Resolution

      00:05

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      Transition from an infinite 2D optical lattice to an axially bound lattice light sheet, as seen in views of the illumination pattern in the rear pupil of the excitation objective (left) and the cross-sectional…

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      • Movie 13 High Resolution

        02:10

        from HHMI NEWS / Added

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        Volume rendering and orthoslices of myosin II highlighting the myosin-rich purse string during dorsal closure in a D. melanogaster embryo, over 1000 time points at 6.0 sec intervals (cf., Fig. 6D).…

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        • Movie 11 High Resolution

          00:22

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          Localization of the chromosomal passenger protein AIR-2 during the first few cell divisions of the early C. elegans embryo (cf., Fig. 6A, fig. S12). Credit: Betzig Lab, HHMI/Janelia Research Campus,…

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          • Movie 10 High Resolution

            00:04

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            Volume rendering from four different viewing angles of a neurophilic HL-60 cell expressing mCherry-utrophin migrating on a 2D glass substrate over 150 time points at 1.1 sec intervals. Credit:…

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            • Movie 7 High Resolution

              01:23

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              Rapid movements of a single cell protozoan T. thermophila expressing GFP-scramblase and confined in a small pocket in agarose (cf., Fig. 4C). Rapid translational movements present in the movie represent…

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              • Movie 9 High Resolution

                00:40

                from HHMI NEWS / Added

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                Two-color volume rendering of a neutrophilic HL-60 cell expressing mCherry-utrophin migrating through a 3D collagen matrix labeled with FITC over 250 time points at 1.3 sec intervals (cf., Fig. 5D-F). Credit:…

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                • Movie 8 High Resolution

                  00:17

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                  Two-color volume rendering seen from two different orientations of the interaction of a T-cell expressing mEmerald-Lifeact (orange) with a target cell expressing a plasma membrane marker fused to…

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                  • Movie 6 High Resolution

                    00:15

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                    Chromosomes (green, mCherry-H2B), mitochondria (yellow, mitotracker – deep red), and endoplasmic reticulum ( magenta, mEmerald-calnexin) in a field of dividing LLC-PK1 cells (cf., Fig. 4B). Left:…

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                    • Movie 5 High Resolution

                      01:02

                      from HHMI NEWS / Added

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                      Measured GFP-EB1 trajectories denoting growing microtubule endpoints, color coded by velocity, and TagRFP-H2B highlighting chromosomes, at five different stages during the division of a single HeLa…

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