Interaction of filamentous actin (mApple-F-tractin, purple) with myosin IIA bipolar head groups (EGFP, myosin IIA, green) at 20-second intervals for 100 time points, as seen with high-NA TIRF-SIM.
Background: Scientists can now watch dynamic biological processes with unprecedented clarity in living cells using new imaging techniques developed by researchers at the Howard Hughes Medical Institute's Janelia Research Campus. The new methods dramatically improve on the spatial resolution provided by structured illumination microscopy, one of the best imaging methods for seeing inside living cells.
EMBARGO: This video and accompanying information are under embargo by the journal Science until Thursday, August 27, 2015 at 2 PM EDT.
Li et. al., “Extended Resolution Structured Illumination Imaging of Endocytic and Cytoskeletal Dynamics,” Science.
More info: hhmi.org/news/imaging-techniques-set-new-standard-super-resolution-live-cells