This video is about ASHG Workshop - Pt3
The accessibility of DNA to proteins such as transcription factors and polymerases has long been a surrogate for regulatory activity. The recent description of the Assay for Transposase Accessible Chromatin sequencing (ATAC-seq) has transformed the analysis of gene regulation by allowing highly efficient generation of sequencing libraries that map regions of DNA accessible to proteins, thus identifying active promoters, enhancers, and other cis-regulatory elements. Unlike other epigenome mapping technologies, ATAC-seq requires 50,000 cells or less and is completed in less than one working day. Using the KAPA Biosystems HiFi Polymerase, an optimized ATAC-seq protocol was developed that efficiently amplifies sequencing libraries from 1,000 – 50,000 cells. These results provide a benchmark for using the ATAC-seq assay on limiting numbers of cells.