Genome engineering with CRISPR systems
Duhee Bang, Yonsei University
Developing new methods for genetic modifications is important for interrogating sequence variants. A recently developed genome-editing method based on RNA-guided clustered regularly interspaced short palindromic repeats (CRISPR)-associated nuclease Cas9 provides innovative technologies for targeted genomic knockout, transcriptional activation, and repression using single-guide RNA. The technology demonstrates scalability by targeting tens of thousands of genomic loci. Using this system, endogenous genome sequences are now easily modified in almost all organisms. Researchers are now utilizing CRISPR system to identify causal links between genetic mutations and biological functions. In this introductory lecture, I will describe the development of CRISPR systems, and a variety of applications in basic research and medicine. Also, I will discuss future directions in the field of genome editing.