Electromagnetic spectrum, Components and functioning of an UV-vis spectrophotomer: Light sources, monochromator, light detectors. Signal to noise ratio, signal modulation, single beam, double beam. What is Beer and Lambert’s Law, Molar absorptivity ( Molar Extinction coefficient) , E 1%, (lambda)Max
How to calculate from Absorbance?
How to calculate concentration of analyte from Absorbance using
and Calibration curve? Application in food analysis
Understand the simple components of a UV-VIS spectrum. Converting percent transmittance to absorbance, or absorbance to percent transmittance. Using a calibration graph to determine the concentration of an unknown sample solution by measuring absorbance/transmittance.