Correlative cryogenic 3D super-resolution and block-face electron microscopy of whole vitreously frozen cells. Two COS-7 cells expressing markers for the endoplasmic reticulum (mEmerald-ER3, green) and mitochondria (Halo/JF535-TOMM20, magenta) are shown in relation to orthoslice (grayscale) or volume- rendered (cyan, plasma membrane; orange, intracellular volume) FIB-SEM data. An ER3-positive intranuclear vesicle and several cytosolic TOMM20-positive vesicles identified by correlation are also highlighted.
For more information: janelia.org/news/new-microscopy-technique-shows-cells-3-d-ultrastructure-in-new-detail
Credit: D. Hoffman et al./Science 2020