Progress of Stem Cell Research and Potential Application in Regenerative Medicine
Qi ZHOU, Institute of Zoology, Chinese Academy of Sciences
Stem Cell, with its property of unlimited proliferation and fully developmental potential, has become important cell resource in regenerative medicine research. For most researches focus on embryonic stem cells, the ethical issue has been an obstacle for its application. Induced pluripotent stem (iPS) technique is not limited by availability of donor cells or ethical objections, so it has been the hotspot of stem cell research and expected to have broad applications in regenerative medicine since it was invented in 2006. Up to now, ES cells and iPS cells have been successfully derived from mice, human, monkeys, rats and pigs and have been differentiated into many tissue specific cells. Recently, several progresses in stem cell research field have been achieved: Using the tetraploid complementation method, we successfully produced viable mice from iPS cells, proven that fully reprogrammed iPS cells possessed similar pluripotency as ES cells; microarray and high-throughput sequencing data revealed that mouse ES and iPS cells with tetraploid complementation ability (4n-iPS cells) shared similar expression patterns, but were distinguishable from tetraploid complementation incompetent iPS cells in the Dlk1-Dio3 imprinting region; we also demonstrated that this marker region was also positively correlated with the pluripotent levels of 3F-iPS cells, indicating the feasibility of using this region as a marker to select full-pluripotent 3F-iPS cells; by comparing the development of embryonic and adult mice derived from iPS cells, we found it exhibited similar developmental features as ES cells, yet were prone to tumorigenesis. These achievements will greatly promote the application of stem cell in both basic research and regenerative medicine.