I made this video to show the different steps of analysis performed on a series of 3D microscope images. The original data, at the beginning, are rapidly acquired 3D confocal micrographs depicting the diffusion of a cluster of 4 microspheres. Each particle is 2 um in diameter and fluorescently labeled. They are permanently aggregated together into a tetrahedral configuration. The tetrahedron is interesting for material scientists for a number of reasons as it's the most basic three-dimensional volume. The video shows the conversion of the 3D micrograph into a 3D reconstruction, which we can then analyze with computer software to find the precise 3D coordinates of each particle. We can use these 3D coordinates to draw computer-generated images of the particles, where the bonds between each particle are drawn for clarity. We can even color the particles since each particle is tracked uniquely, allowing us to accurately measure the cluster's rotational diffusion.