On Wednesday, 22 April 2009, Professor Ichiro Matsumura from Emory University presented a lunchtime talk about using Acinetobacter ADP1 as a Synthetic Biology chassis:
Synthetic biologists generally build upon the Escherichia coli chassis, which usually necessitates a life of cloning. Acinetobacter baylyi sp. ADP1 offers two significant advantages over E. coli: natural competence, and efficient homologous recombination. I will explain how these natural advantages obviate cloning and streamline the directed evolution of genes and genomes. I will also describe the engineering of a broad host range expression vector pBAV1K (Biobrick Accepting Vector) that replicates and produces proteins in a wide variety of gram negative and gram positive bacteria. These tools enable the facile laboratory evolution of genes and chromosomes, without restriction digests or ligation reactions.