A video from the 2014 review by Amy E. Hulme and Thomas J. Hope, "Viral Manipulation of Plant
Host Membranes," from the Annual Review of Virology: annualreviews.org/doi/abs/10.1146/annurev-virology-031413-085502?utm_source=vimeo&utm_medium=vi.hulme&utm_campaign=suppvideo
Detection of viral fusion with live cell imaging. The fusion of Tomato-Vpr/iGFP-labeled HIV-1 with CHO cells was imaged at 37°C under a blood/gas mixture of CO2 on a DeltaVision OMX microscope. The experiment was imaged for 44 min with a Z series of images taken every 45 s. Viral fusion was observed as a decrease in GFP fluorescence after 37.5 min due to release of the fluid phase GFP marker into the cytoplasm of the cell. The Tomato-Vpr-labeled virus retained a lower amount of GFP fluorescence after fusion due to GFP localization to the interior of the capsid (Figure 1). See Figure 2 for further details.
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